LOCUS       sequence-109871-       11797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pCaSpeR4 cyto Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(429..431,432..1145)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(471..492)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(732..751)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1079..1100
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1300..1381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1337..1356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1391..1410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(1445..1646,1647..1677)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2214..2233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2333..2355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2393..2411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2479..2583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2584..2652,2653..3444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2802..2821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3615..4203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4104..4123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4438..4468,4469..5023)
                     /label=P element 5' end
     gene            complement(5034..9169)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     9142..9160
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gcggccgcat ggctcaagag tttgtgaact gcaaaatcca gcctgggaag gtggttgtgt
       61 tcatcaagcc cacctgcccg tactgcagga gggcccaaga gatcctcagt caattgccca
      121 tcaaacaagg gcttctggaa tttgtcgata tcacagccac caaccacact aacgagattc
      181 aagattattt gcaacagctc acgggagcaa gaacggtgcc tcgagtcttt attggtaaag
      241 attgtatagg cggatgcagt gatctagtct ctttgcaaca gagtggggaa ctgctgacgc
      301 ggctaaagca gattggagct ctgcagacta gtggtggttc aggtggtggt ggttcaggtg
      361 gtggtggttc aggtggagga ggatcaggag gaggaggatc aggaggagga ggatcaggag
      421 gagaattcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      481 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      541 cctacggcaa gctgaccctg aagttcatct ccaccaccgg caagctgccc gtgccctggc
      601 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      661 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      721 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      781 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      841 ggcacaagct ggagtacaac tacaactgcc acaacgtcta tatcatggcc gacaagcaga
      901 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
      961 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1021 accactacct gagcacctgc tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1081 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1141 agtaatctag agtcgacctc gaacgttaac gttaacgtaa cgttaactcg agctagatca
     1201 taatcagcca taccacattt gtaaaggttt taccgcgaga aaaaacctcc cacacctccc
     1261 cctgaacctg aaacataact tgtctgcgtt gttgttgtta acttgtttat tgcagcttat
     1321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1381 cattctagtt gtggtttgtc caaactcatc aatgtatgtc gaggtcgacc tgcagcccaa
     1441 gctttgcgta ctcgcaaatt attaaaaata aaactttaaa aataatttcg tctaattaat
     1501 attatgagtt aattcaaacc ccacggacat gctaagggtt aatcaacaat catatcgctg
     1561 tctcactcag actcaatacg acactcagaa tactattcct ttcactcgca cttattgcaa
     1621 gcatacgtta agtggatgtc tcttgccgac gggaccacct tatgttattt catcatggtc
     1681 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     1741 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat
     1801 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     1861 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     1921 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     1981 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     2041 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     2101 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     2161 tagttccgcg cgattcggtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     2221 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     2281 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     2341 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     2401 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     2461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2821 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3121 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3601 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3661 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3721 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3781 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3841 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3901 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3961 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     4021 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4081 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4141 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4201 aaaaacgcct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     4261 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     4321 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     4381 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggcccat
     4441 gatgaaataa cataaggtgg tcccgtcgat agccgaagct taccgaagta tacacttaaa
     4501 ttcagtgcac gtttgcttgt tgagaggaaa ggttgtgtgc ggacgaattt ttttttgaaa
     4561 acattaaccc ttacgtggaa taaaaaaaaa tgaaatattg caaattttgc tgcaaagctg
     4621 tgactggagt aaaattaatt cacgtgccga agtgtgctat taagagaaaa ttgtgggagc
     4681 agagccttgg gtgcagcctt ggtgaaaact cccaaatttg tgatacccac tttaaagatt
     4741 cgcagtggaa ggctgcacct gcaaaaggtc agacatttaa aaggaggcga ctcaacgcag
     4801 atgccgtacc tagtaaagtg atagagcctg aaccagaaaa gataaaagaa ggctatacca
     4861 gtgggagtac acaaacagag taagtttgaa tagtaaaaaa aatcatttat gtaaacaata
     4921 acgtgactgt gcgttaggtc ctgttcattg tttaatgaaa ataagagctt gagggaaaaa
     4981 attcgtactt tggagtacga aatgcgtcgt ttagagcagc agccgaatta attctagttc
     5041 cagtgaaatc caagcatttt ctaaattaaa tgtattctta ttattatagt tgttattttt
     5101 gatatatata aacaacacta ttatgcccac catttttttg agatgcatct acacaaggaa
     5161 caaacactgg atgtcacttt cagttcaaat tgtaacgcta atcactccga acaggtcaca
     5221 aaaaattacc ttaaaaagtc ataatattaa attagaataa atatagctgt gagggaaata
     5281 tatacaaata tattggagca aataaattgt acatacaaat atttattact aatttctatt
     5341 gagacgaaat gaaccactcg gaaccatttg agcgaaccga atcgcgcgga actaacgaca
     5401 gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt gggagacagc
     5461 gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg cgcgcgattt
     5521 gaaaagtatg tatataaaaa atatatcccg gtgttttatg tagcgataaa cgagtttttg
     5581 atgtaaggta tgcaggtgtg taagtctttt ggttagaaga caaatccaaa gtctacttgt
     5641 ggggatgttc gaaggggaaa tacttgtatt ctataggtca tatcttgttt ttattggcac
     5701 aaatataatt acattagctt tttgaggggg caataaacag taaacacgat ggtaataatg
     5761 gtaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg cgtcgggccc
     5821 gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg gccagaccca
     5881 cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg accttgcccg
     5941 aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc acgtccgccc
     6001 attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac gacaaccatt
     6061 tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc aggaatggta
     6121 tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg caggatatta
     6181 gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg cagttgaaga
     6241 agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg aagaccagtg
     6301 gcactgtgag aaaaagcggt aattcggcaa tcgttttgcc cagaaagtat gtgtcacagc
     6361 gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg aacacctaaa
     6421 tcgaatcgat tcattagaaa gttagtaaat tattaatatg caaatgtatt ctaaacaaga
     6481 cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa gaggaagatg
     6541 gctccgttga tattcatcac gcccacttgc gtgagttgtt ggcccaaaaa gatgaggcca
     6601 atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa tattcatatc
     6661 ttcaacatgt tcgcgagtta aatgaaattt atttattttc tgcaaaacta taaactatac
     6721 atctcattga aaaaaactaa gaagggtgtg gaatcaggca attctaacta aaatctagcg
     6781 aatttgtttc caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt
     6841 tgtctgaata agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg
     6901 ccacaggacc gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg
     6961 ctgctccagt ggcttctcca aatttttggt ggccaacaac tgctccatat cccgggctac
     7021 tttgctaata gcaaaattgt cgccatatct tggcgatccg atcacgggac tcgatctccc
     7081 gtccgggcac aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac
     7141 actgggcacc cacgctggat aggagttgag atgtaatgta atgctagata cccttaataa
     7201 acacatcgaa ctcactagga aaagaagtcg acggcttcgc tgggagtgcc caagaaagct
     7261 accctgccct cggccatcag aaggatcttg tcaaagagct caaacagctc ggaagacggc
     7321 tgatgaatgg tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag cacctggacg
     7381 acgctgtggg cggtaaatga gtccagtccg gaggtgggct catcgcagat cagaagcggc
     7441 ggatcggtta gtgcctcgga ggcgaatgcc agacgcttcc tttctccgcc ggacagacct
     7501 ttcaccctgc cgggcacacc gatgatcgtg tgctgacatt tgctgagcga aagctcctgg
     7561 atcacctgat ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg catccgcacc
     7621 atggcctgga aaatcaggtg ttccctggcc gttagggagc cgataaagag gtcatcctgc
     7681 tggacatagg cgcacctggc ctgcatctcc ttggcgtcca caggttggcc attgagcagt
     7741 cgcatcccgg atggcgatac ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc
     7801 agggtcgtct ttccggcacc ggaactgccc atcacggcca aaagttcgcc cggataggcc
     7861 acgccgcaaa ctgagtttca aattggtaat tggacccttt attaagattt cacacagatc
     7921 agccgactgc gaatagaaac tcaccgttct tgagcaaatg tttcctgggc gccggtatgt
     7981 gtcgctcgtt gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg
     8041 gctgattgac cgccccaaag atgtccatat tgtgccaggc ataggtgagg ttctcggcta
     8101 gttggccgct ccctgaaccg gagtcctccg gcggactggg tggcaggagc gtgccgtagt
     8161 ttttggcctg cccgaagccc tggttaatgc agctctgcga agcgtccgct gtcaccctgc
     8221 aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa
     8281 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
     8341 ttaaaatatc tggttgttat attttttgta aaaaagaatg tagtcgaaaa tgaatgcctt
     8401 tagatgtctt gatcatgata tgatcttaaa aattgtctta tatagcgagc acagctacca
     8461 gaataatctg tttcgtgtca ctatttgttt gtgcgattgc ggtttgggat ttttgtgggt
     8521 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
     8581 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
     8641 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
     8701 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
     8761 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
     8821 ttggagcttc gtcacgaaac cgctgacaaa gtgcaactga aggcggacat tgacgctagg
     8881 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
     8941 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
     9001 agaggtaatc gaaagaacct ggtacttcaa atacccttgg atcgaagtaa atttaaaact
     9061 gatcagataa gttcaatgat atccagtgca gtaaaaaata aaaaaaaaat atgttttttt
     9121 aaatctacat tctccaaaaa agggttttat taacttacat acatactaga attcgatatc
     9181 aagcttgcac aggtcctgtt cgataacgtc gtactcggga atcaccttta acttgtccgc
     9241 tattgggtat ttgtccttaa acacattcgt aatctctccc acaatcattg ccctggcctc
     9301 tgcaatgtaa aaagtattta taaaatatcg ctggctctaa agaaaacata ttaatcaaag
     9361 tattgaggct ttatcaatct aacatttaca agtagtcaga ccacaatttt tagactgcac
     9421 ttctgcgtaa gtaaatgaaa tgaaatggtg acctactctt gggtagccgt actgttatat
     9481 gctgacgagc cttcacctgg gcctgctttt tctcctgttt cgtctgatgc gtcggatgcg
     9541 gctggactgg ctgattgttg ggattgggtt ttggatgctg ctccgaaagg tgggccacag
     9601 cttttgcgat ggtggtgccc actatgcggt tgctgcagga actacagctg aatgttcgct
     9661 ttattgtctg cagctcctgg ggctggacga aggccacgcc ctcacccggt gcggccatct
     9721 cctgcggcaa aacgtatgtg ggattcctgt ccaagtagct ctcgatgccc accataatgc
     9781 gctccagcag gttggccagg tgcgggtcta gctgcggcgg attcgtcgtc tggttcactg
     9841 agtttagggt ctgcagaaag aagtgtgcct ccatggtaag cggattcatc tgcacgatca
     9901 gcgggcttta gtctaaaaat tcgaatgata caaactcgta ctcacgtaaa gcagtttgtt
     9961 tggcatcatc tcggccatcg tcttattctt cttcgtggcc tttgttaccg gagcggctat
    10021 gttgggcgcg gtggagcccg gaagctgaaa ggttaatcga atgcgttaac ttttctgcaa
    10081 ctcgaaagtt tgccgccttt gttcgactgc caataactgt tgattcgaaa attcgaatcg
    10141 aagcggttga atttcgtagg gtggccaact acaccaaagt tcgccggcgg tgtatgcatg
    10201 tgagtgtgag tgtgacagtg ggtgtacgtg tgctttaagc gacacaatcg caccgcgatg
    10261 tacatatttt tgtagaaatg tgtgtgcatt tttcgtccgg atcacggctc cataaaataa
    10321 atcatatcac agggctcagg gatttgtgag tagaagttcc cgccacttaa acgtcacttg
    10381 tgaaatcgcc atcgacggtc aacttttact cacactggtc ttccgcggac ggaccgtctc
    10441 aaagtactgc ctttctgcgt tggaaaacat cgcctttttc gtccaaaagg agtccccagg
    10501 ttcgatccgc atggcgttgt gcgtgcgtgc ctttcttttc aaatgattac ggctattaac
    10561 ttgggggcgt taagttggaa acacgtaaat tgcagactgc gattagagtg accatgagta
    10621 ggagttcaaa atctcctgac atcattttct taaaacctgc tttgtttttt acatttctat
    10681 ttaatataac tcctatttga ataaaaaaac aaaacaagtt tagatgttaa gatattaact
    10741 acatcctttg ctccaaaggg agaggggaag ttatggagtt aattaatttg ctgttggaaa
    10801 tcaatatgga gtcagaaata taatgattta ctaaacctta ttgaatcggt aacgatgcga
    10861 atttatatta aaatagcttt tatgaaacat tcaacaaaaa tattattaat gttggcccac
    10921 tttagcaacc ggttaggtct accggttggg caagcaaaga ttcacgccct ggttcgagtc
    10981 ccaactagtc ctgcaaaata ccgcagcaag ttttagagag accaagtgcc attacctctc
    11041 ccacttcagt tatcggttat gcggcgttta agtcgacagc ttgccgtctc tagctccggt
    11101 gcctatataa agcagcccgc tttccacatt tcatattcgt tttacgtttg tcaagcctca
    11161 tagccggcag ttcgaacgta tacgctctct gagtcagacc tcgaaatcgt agctctacac
    11221 aattctgtga attttccttg tcgcgtgtga aacacttcca ataaaaactc ctgcaggtga
    11281 gtactttaaa aaaaaatcta gtgaaataat gctgaaaaga aatttgtgtg ggcaaaattc
    11341 aatgggcaaa aacgcgatgc ggctttttct caaaatggcg gccggcctgc gttttttcct
    11401 caaaagtgat gacgtcatgc ctgttttttt tttttgttcg caatgaggaa tggctcttaa
    11461 aatctactag ataaaaaaaa tattcattat ttctatgctg ctggaacgct tcattaatct
    11521 taaaaattct aaattcggtt accatgatac ttcgacgcat aactgtagat tttggataga
    11581 attaaagaga aaatggcgag agagtaaaat tccggcgtcg gcaaagtaga gcaaaaaaat
    11641 cagtatacca tttagctacc tctctcactc gcacgcagtg ccggctcaag ttgggcgcgg
    11701 ctctgcaatt atcgattttc ttggggtgtg taactaatca tccgttttcc cttcctcctc
    11761 atccacagcg tgaaggtacc cgcccgggat cagatcc
//
