LOCUS       sequence-109872-       11933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 11933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pCaSper4 cyto roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..11933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(315..334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1436..1517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1473..1492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1527..1546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(1581..1782,1783..1813)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2350..2369)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2469..2491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2529..2547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2615..2719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2720..2788,2789..3580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2938..2957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3751..4339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4240..4259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4574..4604,4605..5159)
                     /label=P element 5' end
     gene            complement(5170..9305)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     9278..9296
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gcggccgcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      121 ccacctacgg caagctgacc ctgaagttca tctccaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aactacaact gccacaacgt ctatatcatg gccgacaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagcacc tgctccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagactag tggtggttca ggtggtggtg gttcaggtgg tggtggttca ggtggaggag
      781 gatcaggagg aggaggatca ggaggaggag gatcaggagg agaattcgat atctcagaat
      841 tctataagct agcacctgtt gacaagaaag gccaaccatt ccccttcgac caattaaagg
      901 gaaaagtggt gcttatcgtt aatgttgcct ccaaatgtgg attcactcct caatacaaag
      961 aactagaggc cttgtacaaa cgttataagg acgaaggatt taccatcatc gggttcccat
     1021 gcaaccagtt tggccaccaa gaacctggct ctgatgaaga aattgcccag ttctgccaac
     1081 tgaactatgg cgtgactttc cccattatga aaaaaattga cgttaatggt ggcaatgagg
     1141 accctgttta caagtttttg aagagccaaa aatccggtat gttgggcttg agaggtatca
     1201 aatggaattt tgaaaaattc ttagtcgata aaaagggtaa agtgtacgaa agatactctt
     1261 cactaaccaa accttcttcg ttgtccgaaa ccatcgaaga acttttgaaa gaggtggaat
     1321 agctcgagct agatcataat cagccatacc acatttgtaa aggttttacc gcgagaaaaa
     1381 acctcccaca cctccccctg aacctgaaac ataacttgtc tgcgttgttg ttgttaactt
     1441 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1501 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatgtcgagg
     1561 tcgacctgca gcccaagctt tgcgtactcg caaattatta aaaataaaac tttaaaaata
     1621 atttcgtcta attaatatta tgagttaatt caaaccccac ggacatgcta agggttaatc
     1681 aacaatcata tcgctgtctc actcagactc aatacgacac tcagaatact attcctttca
     1741 ctcgcactta ttgcaagcat acgttaagtg gatgtctctt gccgacggga ccaccttatg
     1801 ttatttcatc atggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     1861 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     1921 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     1981 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     2041 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     2101 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     2161 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     2221 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     2281 ttggagcgac tgtcgttagt tccgcgcgat tcggtgcggt atttcacacc gcatatggtg
     2341 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     2401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2941 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3241 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3421 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4141 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4321 ggggcggagc ctatggaaaa acgccttctt cttgaactcg ggctcggtgc cagtatacct
     4381 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
     4441 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
     4501 ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
     4561 cgtgggtctg gcccatgatg aaataacata aggtggtccc gtcgatagcc gaagcttacc
     4621 gaagtataca cttaaattca gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac
     4681 gaattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa atattgcaaa
     4741 ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg tgctattaag
     4801 agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca aatttgtgat
     4861 acccacttta aagattcgca gtggaaggct gcacctgcaa aaggtcagac atttaaaagg
     4921 aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc agaaaagata
     4981 aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt aaaaaaaatc
     5041 atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta atgaaaataa
     5101 gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag agcagcagcc
     5161 gaattaattc tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat
     5221 tatagttgtt atttttgata tatataaaca acactattat gcccaccatt tttttgagat
     5281 gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca
     5341 ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat
     5401 agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt
     5461 attactaatt tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg
     5521 cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga
     5581 gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga
     5641 agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc
     5701 gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa
     5761 tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc
     5821 ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa
     5881 cacgatggta ataatggtaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac
     5941 tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg
     6001 agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc
     6061 aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc
     6121 ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag
     6181 aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa gaagccgcca
     6241 aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga ggtcgaggag
     6301 ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag agtgaccagc
     6361 gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg ataggcaatc
     6421 gccgtgaaga ccagtggcac tgtgagaaaa agcggtaatt cggcaatcgt tttgcccaga
     6481 aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac tggcagctct
     6541 gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt aatatgcaaa
     6601 tgtattctaa acaagactta catttatcgt ggcaaagacg ttttgaaagg tcatgttggt
     6661 caggaagagg aagatggctc cgttgatatt catcacgccc acttgcgtga gttgttggcc
     6721 caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt actcgcatct
     6781 cattaatatt catatcttca acatgttcgc gagttaaatg aaatttattt attttctgca
     6841 aaactataaa ctatacatct cattgaaaaa aactaagaag ggtgtggaat caggcaattc
     6901 taactaaaat ctagcgaatt tgtttccaag aattgtaagc gttatatcat ttgtttccac
     6961 tggaaccact caccgttgtc tgaataagtc gcacttttac gaggagtggt tccttgagca
     7021 ccgacagcca ggatcgccac aggaccgccc ggaactgcat gaaccaggtg gccttgtagg
     7081 tgtacccatt ctccggctgc tccagtggct tctccaaatt tttggtggcc aacaactgct
     7141 ccatatcccg ggctactttg ctaatagcaa aattgtcgcc atatcttggc gatccgatca
     7201 cgggactcga tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa gtccgccgga
     7261 ttgtagttgg taggacactg ggcacccacg ctggatagga gttgagatgt aatgtaatgc
     7321 tagataccct taataaacac atcgaactca ctaggaaaag aagtcgacgg cttcgctggg
     7381 agtgcccaag aaagctaccc tgccctcggc catcagaagg atcttgtcaa agagctcaaa
     7441 cagctcggaa gacggctgat gaatggtcag gatgacggtc ttgcccttct gcgacagctt
     7501 cttcagcacc tggacgacgc tgtgggcggt aaatgagtcc agtccggagg tgggctcatc
     7561 gcagatcaga agcggcggat cggttagtgc ctcggaggcg aatgccagac gcttcctttc
     7621 tccgccggac agacctttca ccctgccggg cacaccgatg atcgtgtgct gacatttgct
     7681 gagcgaaagc tcctggatca cctgatccac gcgggccact cgctgccgat aggtcagatg
     7741 tcgtggcatc cgcaccatgg cctggaaaat caggtgttcc ctggccgtta gggagccgat
     7801 aaagaggtca tcctgctgga cataggcgca cctggcctgc atctccttgg cgtccacagg
     7861 ttggccattg agcagtcgca tcccggatgg cgatacttgg atgccctgcg gcgatcgaaa
     7921 ggcaagggca ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca cggccaaaag
     7981 ttcgcccgga taggccacgc cgcaaactga gtttcaaatt ggtaattgga ccctttatta
     8041 agatttcaca cagatcagcc gactgcgaat agaaactcac cgttcttgag caaatgtttc
     8101 ctgggcgccg gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc
     8161 cgccatccgg agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag
     8221 gtgaggttct cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc
     8281 aggagcgtgc cgtagttttt ggcctgcccg aagccctggt taatgcagct ctgcgaagcg
     8341 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     8401 ctaaccccga acaaaacgaa gtatcctacg aagtaggttt atacttttat ttattttttg
     8461 tgcatctagg atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatgtagt
     8521 cgaaaatgaa tgcctttaga tgtcttgatc atgatatgat cttaaaaatt gtcttatata
     8581 gcgagcacag ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc gattgcggtt
     8641 tgggattttt gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc aatcgcagtt
     8701 cctatagatc aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct
     8761 cggcagatgg gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg
     8821 ccgggatttt tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac
     8881 tcaaaaaaca aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat
     8941 tgatggcgta aaccgcttgg agcttcgtca cgaaaccgct gacaaagtgc aactgaaggc
     9001 ggacattgac gctaggtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg
     9061 aaagagacgg cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg
     9121 aatgtcattt tgagtgagag gtaatcgaaa gaacctggta cttcaaatac ccttggatcg
     9181 aagtaaattt aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaataaaaa
     9241 aaaaatatgt ttttttaaat ctacattctc caaaaaaggg ttttattaac ttacatacat
     9301 actagaattc gatatcaagc ttgcacaggt cctgttcgat aacgtcgtac tcgggaatca
     9361 cctttaactt gtccgctatt gggtatttgt ccttaaacac attcgtaatc tctcccacaa
     9421 tcattgccct ggcctctgca atgtaaaaag tatttataaa atatcgctgg ctctaaagaa
     9481 aacatattaa tcaaagtatt gaggctttat caatctaaca tttacaagta gtcagaccac
     9541 aatttttaga ctgcacttct gcgtaagtaa atgaaatgaa atggtgacct actcttgggt
     9601 agccgtactg ttatatgctg acgagccttc acctgggcct gctttttctc ctgtttcgtc
     9661 tgatgcgtcg gatgcggctg gactggctga ttgttgggat tgggttttgg atgctgctcc
     9721 gaaaggtggg ccacagcttt tgcgatggtg gtgcccacta tgcggttgct gcaggaacta
     9781 cagctgaatg ttcgctttat tgtctgcagc tcctggggct ggacgaaggc cacgccctca
     9841 cccggtgcgg ccatctcctg cggcaaaacg tatgtgggat tcctgtccaa gtagctctcg
     9901 atgcccacca taatgcgctc cagcaggttg gccaggtgcg ggtctagctg cggcggattc
     9961 gtcgtctggt tcactgagtt tagggtctgc agaaagaagt gtgcctccat ggtaagcgga
    10021 ttcatctgca cgatcagcgg gctttagtct aaaaattcga atgatacaaa ctcgtactca
    10081 cgtaaagcag tttgtttggc atcatctcgg ccatcgtctt attcttcttc gtggcctttg
    10141 ttaccggagc ggctatgttg ggcgcggtgg agcccggaag ctgaaaggtt aatcgaatgc
    10201 gttaactttt ctgcaactcg aaagtttgcc gcctttgttc gactgccaat aactgttgat
    10261 tcgaaaattc gaatcgaagc ggttgaattt cgtagggtgg ccaactacac caaagttcgc
    10321 cggcggtgta tgcatgtgag tgtgagtgtg acagtgggtg tacgtgtgct ttaagcgaca
    10381 caatcgcacc gcgatgtaca tatttttgta gaaatgtgtg tgcatttttc gtccggatca
    10441 cggctccata aaataaatca tatcacaggg ctcagggatt tgtgagtaga agttcccgcc
    10501 acttaaacgt cacttgtgaa atcgccatcg acggtcaact tttactcaca ctggtcttcc
    10561 gcggacggac cgtctcaaag tactgccttt ctgcgttgga aaacatcgcc tttttcgtcc
    10621 aaaaggagtc cccaggttcg atccgcatgg cgttgtgcgt gcgtgccttt cttttcaaat
    10681 gattacggct attaacttgg gggcgttaag ttggaaacac gtaaattgca gactgcgatt
    10741 agagtgacca tgagtaggag ttcaaaatct cctgacatca ttttcttaaa acctgctttg
    10801 ttttttacat ttctatttaa tataactcct atttgaataa aaaaacaaaa caagtttaga
    10861 tgttaagata ttaactacat cctttgctcc aaagggagag gggaagttat ggagttaatt
    10921 aatttgctgt tggaaatcaa tatggagtca gaaatataat gatttactaa accttattga
    10981 atcggtaacg atgcgaattt atattaaaat agcttttatg aaacattcaa caaaaatatt
    11041 attaatgttg gcccacttta gcaaccggtt aggtctaccg gttgggcaag caaagattca
    11101 cgccctggtt cgagtcccaa ctagtcctgc aaaataccgc agcaagtttt agagagacca
    11161 agtgccatta cctctcccac ttcagttatc ggttatgcgg cgtttaagtc gacagcttgc
    11221 cgtctctagc tccggtgcct atataaagca gcccgctttc cacatttcat attcgtttta
    11281 cgtttgtcaa gcctcatagc cggcagttcg aacgtatacg ctctctgagt cagacctcga
    11341 aatcgtagct ctacacaatt ctgtgaattt tccttgtcgc gtgtgaaaca cttccaataa
    11401 aaactcctgc aggtgagtac tttaaaaaaa aatctagtga aataatgctg aaaagaaatt
    11461 tgtgtgggca aaattcaatg ggcaaaaacg cgatgcggct ttttctcaaa atggcggccg
    11521 gcctgcgttt tttcctcaaa agtgatgacg tcatgcctgt tttttttttt tgttcgcaat
    11581 gaggaatggc tcttaaaatc tactagataa aaaaaatatt cattatttct atgctgctgg
    11641 aacgcttcat taatcttaaa aattctaaat tcggttacca tgatacttcg acgcataact
    11701 gtagattttg gatagaatta aagagaaaat ggcgagagag taaaattccg gcgtcggcaa
    11761 agtagagcaa aaaaatcagt ataccattta gctacctctc tcactcgcac gcagtgccgg
    11821 ctcaagttgg gcgcggctct gcaattatcg attttcttgg ggtgtgtaac taatcatccg
    11881 ttttcccttc ctcctcatcc acagcgtgaa ggtacccgcc cgggatcaga tcc
//
