LOCUS       sequence-109884-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G35E mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)G35E
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(620..640)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(644..661)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc tttcacgggg
       61 acatcacctc ctttggctgg gctttcttgc attccaccat tttgttgttg atttcatgga
      121 agtggatctc acaaactttc tctacgatgt cctcgctctc aaacgtgaca aatccaaacc
      181 ctctgtgcct gttggtggtt ttgtcgaaca tcagcatggc atcatccacc tttccgaact
      241 gctcgaaata gtgtttcaca tcttccaccg tggtgttcac agacagcccc cccacgaaga
      301 tcttcttcgt ccgagtgacc atcttaggct gtgctcttcg aggaaaggcc accttggggt
      361 caattgtttt ggagtcgagc tcgtgccgcg attgcgccag cactttatcc acccccgcct
      421 ggtccatgaa agtgacgaag ccgaaacccc tggatctttt ggtcaggggg tcccgcatca
      481 ccagacactc tttcacctcc ccgaactggc cgaagtattc gcgcagttct tcctgcgtgg
      541 tctgccaact gagtcctccg atgaacatct tgcaggggtc gtgcggcgag tccggggagg
      601 cgaggccggg ctgggatccg ccctgaaaat acaggttttc gccgtgatga tgatgatgat
      661 gcatatgtat atctc
//
