LOCUS       sequence-109887-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H83F mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)H83F
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(613..633)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agctttcacg gggacatcac
       61 ctcctttggc tgggctttct tgcattccac cattttgttg ttgatttcat ggaagtggat
      121 ctcacaaact ttctctacga tgtcctcgct ctcaaacgtg acaaatccaa accctctgtg
      181 cctgttggtg gttttgtcga acatcagcat ggcatcatcc acctttccga actgctcgaa
      241 atagtgtttc acatcttcca ccgtggtgtt cacagacagc ccccccacga agatcttctt
      301 cgtccgagtg accatcttag gctgtgctct tcgaggaaag gccaccttgg ggtcaattgt
      361 tttggagtcg agctcaaacc gagattgcgc cagcacttta tccacccccg cctggtccat
      421 gaaagtgacg aagccgaaac ccctggatct tttggtcagg gggtcccgca tcaccagaca
      481 ctctttcacc tccccgaact ggccgaagta ttcgcgcagc ccttcctgcg tggtctgcca
      541 actgagtcct ccgatgaaca tcttgcaggg gtcgtgcggc gagtccgggg aggcgaggcc
      601 gggctgggat ccgccctgaa aatacaggtt ttcgccgtga tgatgatgat gatgcatatg
      661 tatatctcct tcttaaagtt
//
