LOCUS       sequence-109889-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R53E R59E R61E triple mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)R53E/R59E/R61E
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(615..635)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(639..656)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagctttca cggggacatc
       61 acctcctttg gctgagcttt cttgcattcc accattttgt tgttgatttc atggaagtgg
      121 atctcacaca ctttctctac gatgtcctca ctctcgaacg tgacaaaccc gaaccctctg
      181 tgcctgttgg tggttttgtc gaacatcagc atggcatcat ccacctttcc gaactgctca
      241 aaatagtgct tcacgtcctc caccgtggtg ttcacagaca gcccccccac aaagatcttc
      301 ttagttcgag tgaccatctt aggctgtgct ctccgaggaa aggccacctt ggggtcaatt
      361 gttttggagt cgagctcgtg ccgagattgc gccagcactt tatccacccc cgcctggtcc
      421 atgaaagtga cgaagccgaa accctcggac tctttggtca gggggtcctc catcaccaga
      481 cactctttca cctccccgaa ctggccgaag tattcgcgca gcccttcctg cgtggtctgc
      541 caactgagtc ctccgatgaa catcttgcag gggtcgtgcg gcgagtccgg ggaggcgagg
      601 ccgggctggg atccgccctg aaaatacagg ttttcgccgt gatgatgatg atgatgcata
      661 tgtatatctc cttc
//
