LOCUS       sequence-109890-         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the RBD of human MSI2 in bacterial cells for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI2(8-193)
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagctttca tgggaacatg
       61 acttctttcg gctgagcttt cttacattct accattttat tattgatttc atggaaatga
      121 atctcacaga ctttctccac aacatcttca ttctcaaaag tgacaaagcc aaaccctctg
      181 tgcctgttgg tagttttatc aaacatcagc attgcatctt ccaccttgcc aaactgctcg
      241 aaatattgct ttacatcttc cactactgtg ttcgcagata acccgcctac aaatattttc
      301 tttgttctcg tgaccatctt gggttgcgct cgacgaggaa atgcaacttt ggggtcaatc
      361 gtcttggaat ctaactcatg gtggggctga cctaatactt tatctacact tgctgggtct
      421 gcgaacgtga cgaaaccgaa gcctctggag cgtttcgtag tgggatctct catgaccata
      481 cattctctaa tttctccaaa tttgctaaaa tagtctctaa ggctatctgg tgaggtctgc
      541 cagctcagtc caccgataaa cattttaccg gggtcgtgct gggagtcgtt ggcgctgccc
      601 gaggtgccgg atccgccctg aa
//
