LOCUS       sequence-109891-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN mediated gene editing at human TUBA1B locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, 
            Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, 
            Danuser G
  TITLE     Vimentin Intermediate Filaments Template Microtubule Networks to 
            Enhance Persistence in Cell Polarity and Directed Migration.
  JOURNAL   Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007.
  PUBMED    27667364
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007."
COMMENT     Sequence Label: pGoldyTALEN-TUBA1B-TAL-2
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..72
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             73..99
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 gatattgtat atatcgtaac aataggaggt tcaacaatgg cttcctcccc tccaaagaaa
       61 aagagaaagg ttagttggaa ggacgcaagt ggttggtcta gagtggatct acgcacgctc
      121 ggctacagtc agcagcagca agagaagatc aaaccgaagg tgcgttcgac agtggcgcag
      181 caccacgagg cactggtggg ccatgggttt acacacgcgc acatcgttgc gctcagccaa
      241 cacccggcag cgttagggac cgtcgctgtc acgtatcagc acataatcac ggcgttgcca
      301 gaggcgacac acgaagacat cgttggcgtc ggcaaacagt ggtccggcgc acgcgccctg
      361 gaggccttgc tcacggatgc gggggagttg agaggtccgc cgttacagtt ggacacaggc
      421 caacttgtga agattgcaaa acgtggcggc gtgaccgcaa tggaggcagt gcatgcatcg
      481 cgcaatgcac tgacgggtgc ccccctgaac ctgaccccgg accaagtggt ggctatcgcc
      541 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
      601 caggaccatg gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggc
//
