LOCUS       sequence-109896-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4ROH
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(430..448)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      444..491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(615..634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcgaatttg atcctcttgg tccattgcca cctggatggg agaagagaac agacagcaat
      121 ggcagagtat atttcgtcaa ccacaacaca cgaattacac aatgggaaga ccccagaagt
      181 caaggtcaat taaatgaaaa gcccttacct gaaggttggg aaatgagatt cacagtggat
      241 ggaattccat attttgtgga ccacaataga agaactacca cctatataga tccccgcaca
      301 ggaaaatctg ccctagacaa tggacctcag tgatgacgaa gcttgcggcc gcactcgagc
      361 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      421 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      481 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      661 tccccgtcaa gctctaaatc
//
