LOCUS       sequence-109898-        4343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli reporter vector with MCS for inserting bacterial promoter 
            region through 5' end of gene of interest, upstream and in-frame 
            with reporter cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPReporter
FEATURES             Location/Qualifiers
     source          1..4343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      439..894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(526..545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     736..757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(1009..1800,1801..1869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1632..1651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1870..1962)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2043..2631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2532..2551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2785..2802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2817..2959
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2885..2904)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3045..3067
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3061..3252)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gggcccgctg ggagttcgta gacggaccta ggtctagatt cagccaaaaa acttaagacc
       61 gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt tttcttttct
      121 cttctcaaca ttactcgcat ccattctcgg cgatcgcgcg gccgctttaa ttaagaattc
      181 aagcttagat ctcaattgct cgagggcggt ggcggtagtg gcggtggcgg ttccggcggt
      241 ggcggttcac atatggcact gaaggtcctc aatcgcactg gaaacatcaa ggtcgggtac
      301 cggaaacaca gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg accaaaggag
      361 gctgtctcgt ctcgtctcac tagtgctagc aacaaacgca gaatccaagc gtcgactata
      421 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      481 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      541 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      601 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      661 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      721 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      781 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      841 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttctggcg
      901 gcacgatggc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1861 aatactcata ctcttccttt ttcaatcatg attgaagcat ttatcagggt tattgtctca
     1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggtcat gaccaaaatc
     1981 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2041 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2101 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2161 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2221 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2281 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2341 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2401 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2461 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2521 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2581 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2641 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2701 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2761 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     2821 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc
     2881 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2941 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3001 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3061 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     3121 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     3181 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     3241 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     3301 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     3361 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     3421 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     3481 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     3541 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     3601 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     3661 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc
     3721 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     3781 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     3841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3901 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     3961 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     4021 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     4081 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
     4141 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     4201 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     4261 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     4321 gacgcgccga gacagaactt aat
//
