LOCUS       sequence-109901-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgACTA2 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Yu C, Liu Y, Ma T, Liu K, Xu S, Zhang Y, Liu H, La Russa M, Xie M, 
            Ding S, Qi LS
  TITLE     Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem 
            Cells.
  JOURNAL   Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 
            10.1016/j.stem.2015.01.003.
  PUBMED    25658371
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.01"; journalName: "Cell Stem Cell"; date: 
            "2015-02-5- 5"; volume: "16"; issue: "2"; pages: "142-7"
COMMENT     Sequence Label: pX330_sgACTA2
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        388..672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccgcggt ggacaatgga aggccgttta agagctatgc tggaaacagc atagcaagtt
      241 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
      301 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt
      361 ctgcagacaa atggctctag aggtacccgt tacataactt acggtaaatg gcccgcctgg
      421 ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca
      481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcattg
      601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      661 cgctattacc at
//
