LOCUS       sequence-109904-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing P2A_NLS_sfGFP with homologous arm of Nanog..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Yu C, Liu Y, Ma T, Liu K, Xu S, Zhang Y, Liu H, La Russa M, Xie M, 
            Ding S, Qi LS
  TITLE     Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem 
            Cells.
  JOURNAL   Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 
            10.1016/j.stem.2015.01.003.
  PUBMED    25658371
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.01"; journalName: "Cell Stem Cell"; date: 
            "2015-02-5- 5"; volume: "16"; issue: "2"; pages: "142-7"
COMMENT     Sequence Label: pP2A_NLS_sfGFP
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..63)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(70..90)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(103..129)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(109..135)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(142..198)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gctggatccg ctgctgccgt tgctcccact accaatgccg tccactttcc gctttttctt
       61 aggatcttcc accttgcgtt ttttcttggg ggagccgctg cccaggctgg cataatcggg
      121 cacgtcatag ggataggcca taggtccagg gttctcctcc acgtctccag cctgcttcag
      181 caggctgaag ttagtagcta tttcacctgg tggagtcaca gagtagttca ggaataattc
      241 caaggcttgt ggggtgctaa aatgcgcatg gctttcccta gtggcttcca aattcacctc
      301 caaatcactg gcagagaagt tttgctgcaa ctgtacgtaa ggctgcagaa agtcctcccc
      361 gaagttatgg agcggagcag cattccaagg ctggccgttc caggactgag cggtccaggc
      421 ctggctgctc caagttgggt tggtccaggt ctggttgttc caagttgggt tggtccaggt
      481 ctggctgctc caagttgggt tggtccaagt ctggctgccc cacatggaaa ggcttccaga
      541 tgcgttcacc agatagccct ggggatagct gcaatggatg ctgggatact ccactggtgc
      601 tgagcccttc tg
//
