LOCUS       sequence-109906-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgNanog in mammalian cells of mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Yu C, Liu Y, Ma T, Liu K, Xu S, Zhang Y, Liu H, La Russa M, Xie M, 
            Ding S, Qi LS
  TITLE     Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem 
            Cells.
  JOURNAL   Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 
            10.1016/j.stem.2015.01.003.
  PUBMED    25658371
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.01"; journalName: "Cell Stem Cell"; date: 
            "2015-02-5- 5"; volume: "16"; issue: "2"; pages: "142-7"
COMMENT     Sequence Label: pSLQ1371_sgNanog
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(240..273)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(382..402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
       61 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      121 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      181 ccgccactcc accggcggca tggacgagct gtacaagtaa gaattcgtcg agggacctaa
      241 taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta
      301 ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
      361 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
      421 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
      481 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
      541 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
      601 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
      661 tgcccgctgc tggaca
//
