LOCUS       sequence-109920-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV dual reporter vector expressing mCh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Lassen KG, Hebbeler AM, Bhattacharyya D, Lobritz MA, Greene WC
  TITLE     A flexible model of HIV-1 latency permitting evaluation of many 
            primary CD4 T-cell reservoirs.
  JOURNAL   PLoS One. 2012;7(1):e30176. doi: 10.1371/journal.pone.0030176. Epub 
            2012 Jan 24.
  PUBMED    22291913
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1):e30176. doi"; pages: " 
            10.1371/journal.pone.0030176. Epub 2012 Jan 24"
COMMENT     Sequence Label: NL4-3 mCherry Luciferase
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(365..384)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     634..652
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cctttcttta tgtttttggc gtcttctcga gggccgggat tctcctccac gtcaccgcat
       61 gttagaagac ttcctctgcc ctcccggggc ttgtacagct cgtccatgcc gccggtggag
      121 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agccctcgtt gtgggaggtg
      181 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
      241 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
      301 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
      361 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
      421 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
      481 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
      541 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
      601 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
      661 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
//
