LOCUS       sequence-109922-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral golden gate cloning vector for expression of functional 
            Human T-Cell Receptor genes with NGFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE
  TITLE     Production of retroviral constructs for effective transfer and 
            expression of T-cell receptor genes using Golden Gate cloning.
  JOURNAL   Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. 
            eCollection 2015.
  PUBMED    25757546
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114265";
            journalName: "Biotechniques"; date: "2015-03-1- 1"; volume: "58"; 
            issue: "3"; pages: "135-9"
COMMENT     Sequence Label: MSGV Hu Acceptor PGK-NGFR
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgacctgcag gcggccgcac catgggggca ggtgccactg gccgcgccat ggacgggccg
       61 cgcctgctgc tgttgctgct tctgggggtg tcccttggag gtgccaagga ggcatgcccc
      121 acaggcctgt acacacacag cggtgagtgc tgcaaagcct gcaacctggg cgagggtgtg
      181 gcccagcctt gtggagccaa ccagaccgtg tgtgagccct gcctggacag cgtgacgttc
      241 tccgacgtgg tgagcgcgac cgagccgtgc aagccgtgca ccgagtgcgt ggggctccag
      301 agcatgtcgg cgccatgcgt ggaggccgac gacgccgtgt gccgctgcgc ctacggctac
      361 taccaggatg agacgactgg gcgctgcgag gcgtgccgcg tgtgcgaggc gggctcgggc
      421 ctcgtgttct cctgccagga caagcagaac accgtgtgcg aggagtgccc cgacggcacg
      481 tattccgacg aggccaacca cgtggacccg tgcctgccct gcaccgtgtg cgaggacacc
      541 gagcgccagc tccgcgagtg cacacgctgg gccgacgccg agtgcgagga gatccctggc
      601 cgttggatta cacggtccac acccccagag ggctcggaca gcacag
//
