LOCUS       sequence-109925-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4XCX
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcaattttg aagaagttcc cagggagacg gcaattcagt ttaaacctcc actatacaga
      121 cagcggtacc agttcgttaa aaatttagtg gatcaacatg agcctaagaa ggttgcagac
      181 ctgggatgtg gtgatacttc actcttaagg ctgctaaaag tcaatccatg cattgaattg
      241 cttgttggag tagatattaa tgaggataaa ttacgatgga gaggggattc gttagctcct
      301 ttcctggggg attttctgaa acctcgggat ctgaatttga ccatcacatt gtatcatggc
      361 tccgttgtgg agagagactc tcgtttgctt ggatttgact tgataacgtg tattgaatta
      421 atagaacatt tggattcagg tgatctggcc agatttcctg aagtggtatt tgggtacctg
      481 tctccatcca tgattgtcat cagcacacca aactctgaat tcaatcccct gtttccatca
      541 gtgaccttaa gagattcaga tcataaattt gagtggacca gaatggagtt tcagacctgg
      601 gctttatatg tggcaaatcg ctatgattac tctgtggagt ttactggtgt cggggaacca
      661 ccagctggag ctgagaatgt tggatactgt acccagatag gaatcttccg g
//
