LOCUS       sequence-109927-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RG1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcgcagaga aggaggaccg cgggcggccc tacacactga gcgtagccct gccgggctcc
      121 atcctggaca atgctcagtc gccggagctt cgcacctact tggccggtca gattgccaga
      181 gcctgtgcca tcttctgtgt ggatgagatc gtggtgtttg atgaggaggg ccaggatgcc
      241 aagactgtgg agggggaatt cacaggagtt gggaagaagg ggcaggcgtg cgtacagctg
      301 gcccggatcc tgcagtacct ggagtgtcca cagtacctga ggaaggcgtt cttccccaag
      361 caccaggatc tacagtttgc agggctcctg aaccccctgg acagccccca ccacatgcgt
      421 caggatgagg aatccgagtt ccgagagggc atcgtggtgg atcggcccac ccggccaggc
      481 cacggctcct ttgtcaactg tggcatgaaa aaggaggtga agattgacaa gaacctggag
      541 cccgggcttc gggtgactgt gcgactgaac cagcagcagc acccagactg caagacctac
      601 catggcaaag tggtatcatc gcaggaccct cgcaccaaag ctggtctcta ctggggctac
      661 accgtccgac tggcttcctg cctcagtgct gtgtttgctg aggccccctt ccaagatggg
      721 tatgacctga ccatcgggac gtcagagcgc ggctcagatg tggcctctg
//
