LOCUS       sequence-109929-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tobacco etch virus protease (TEV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   To TL, Piggott BJ, Makhijani K, Yu D, Jan YN, Shu X
  TITLE     Rationally designed fluorogenic protease reporter visualizes 
            spatiotemporal dynamics of apoptosis in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 
            10.1073/pnas.1502857112. Epub 2015 Mar 2.
  PUBMED    25733847
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1502857112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3338-43"
COMMENT     Sequence Label: pcDNA3.1 TEV (full-length)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt gccaccatgg gcgagagcct tttcaagggc ccgagggact
      121 acaacccgat ctccagcacc atctgtcacc tgaccaacga gagcgacggt cacaccacta
      181 gtctgtacgg catcggcttc ggccccttca tcatcaccaa caagcatctg ttcaggagga
      241 ataacggcac actgctggtg caaagcctgc acggcgtgtt caaagtgaag aacacaacca
      301 ccctgcaaca gcacctgatc gacggcaggg acatgattat catcaggatg cccaaggact
      361 tccccccctt tccccagaaa ctgaagttca gggagccaca aagggaggag cgaatctgcc
      421 tggtgaccac caacttccag accaagtcca tgagcagcat ggtctctgat accagctgca
      481 ccttccccag cagcgacggc atcttctgga agcactggat tcagacgaag gatggccaat
      541 gcggcagccc attggtgagc actagggacg gcttcatcgt gggcatccac agcgccagca
      601 attttaccaa taccaacaac tacttcacga gcgtgccgaa aaacttcatg gagctgttga
      661 ccaatcaaga ggcgcagcag tgggtgagcg gctggaggct gaacgccgac agcgttcttt
      721 ggggcggaca taaggtgttc atggtcaagc ccgaggaacc cttccagccc gttaaggaag
      781 ccactcagct ttgataactc gagtctagag ggcccgcggt tcgaacaaaa actcatctca
      841 gaa
//
