LOCUS       sequence-109938-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4X3G
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             669..686
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcgctaatt cagtactttt cccctgtaaa tatgcgtctt ctggatgtga aataactctg
      121 ccacacacag aaaaagcaga ccatgaagag ctctgtgagt ttaggcctta ttcctgtccg
      181 tgccctggtg cttcctgtaa atggcaaggc tctctggatg ctgtaatgcc ccatctgatg
      241 catcagcata agtccattac aaccctacag ggagaggata tagtttttct tgctacagac
      301 attaatcttc ctggtgctgt tgactgggtg atgatgcagt cctgttttgg ctttcacttc
      361 atgttagtct tagagaaaca ggaaaaatac gatggtcacc agcagttctt cgcaatcgta
      421 cagctgatag gaacacgcaa gcaagctgaa aattttgctt accgacttga gctaaatggt
      481 cataggcgac gattgacttg ggaagcgact cctcgatcta ttcatgaagg aattgcaaca
      541 gccattatga atagcgactg tctagtcttt gacaccagca ttgcacagct ttttgcagaa
      601 aatggcaatt taggcatcaa tgtaactatt tccatgtgtt gatgacgaag cttgcggccg
      661 cactcgagca ccaccaccac caccactgag atccggctgc taac
//
