LOCUS       sequence-109940-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RG2
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             470..487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(540..558)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      554..601
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaggagata taccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt
       61 tccagggcgg aaatagcttt gtagggctcc gtgttgtagc caagtggtca tccaatggct
      121 acttttactc tgggaaaatc acacgagatg tcggagctgg gaagtataaa ttgctctttg
      181 atgatgggta cgaatgtgat gtgttgggca aagacattct gttatgtgac cccatcccgc
      241 tggacactga agtgacggcc ctctcggagg atgagtattt cagtgcagga gtggtgaaag
      301 gacataggaa ggagtctggg gaactgtact acagcattga aaaagaaggc caaagaaagt
      361 ggtataagcg aatggctgtc atcctgtcct tggagcaagg aaacagactg agagagcagt
      421 atgggcttgg cccctatgaa tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      481 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      541 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      601 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      721 cccgctcctt tcgc
//
