LOCUS       sequence-109941-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses infrared fluorescent executioner caspase reporter 
            (iCasper) plus human heme oxygenase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   To TL, Piggott BJ, Makhijani K, Yu D, Jan YN, Shu X
  TITLE     Rationally designed fluorogenic protease reporter visualizes 
            spatiotemporal dynamics of apoptosis in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 
            10.1073/pnas.1502857112. Epub 2015 Mar 2.
  PUBMED    25733847
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1502857112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3338-43"
COMMENT     Sequence Label: pcDNA3.1 iCasper T2A HO1
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..737
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ccaccatgtc caaaggagaa gaactgttta
      121 ccggtgttgt gccaattttg gttgaactcg atggtgatgt caacggacat aagttctcag
      181 tgagaggcga aggagaaggt gacgccacca ttggaaaatt gactcttaaa ttcatctgta
      241 ctactggtaa acttcctgta ccatggccga ctctcgtaac aacgcttacg tacggagttc
      301 agtgcttttc gagataccca gaccatatga aaagacatga cttttttaag tcggctatgc
      361 ctgaaggtta cgtgcaagaa agaacaattt cgttcaaaga tgatggaaaa tataaaacta
      421 gagcagttgt taaatttgaa ggagatactt tggttaaccg cattgaactg aaaggaacag
      481 attttaaaga agatggtaat attcttggac acaaactcga atacaatttt aatagtcata
      541 acgtatacat cactgctgat aagcaaaaga acggaattaa agcgaatttc acagtacgcc
      601 ataatgtaga agatggcagt gttcaacttg ccgaccatta ccaacaaaac acccctattg
      661 gagacggtcc ggtacttctt cctgataatc actacctctc aacacaaaca gtcctgagca
      721 aagatccaaa tgaaaaagga ggcagcggat ccaccactaa cattgcgccg gctctggacg
      781 gtgcgtttca tcgtatcact tcttcatcct ccctgatggg cctgtgtgac gaaaccgcga
      841 ctattatccg tgagattact ggctacgacc gtgtgatggt agtacgtttc gatgaagagg
      901 gtaatgccga aattctgtcc gaacgtcgtc gtgcggacct ggaagcgttc ct
//
