LOCUS       sequence-109955-         558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus 
            aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA5
FEATURES             Location/Qualifiers
     source          1..558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..51
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             58..102
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtctggtgc cacgcggttc tggtatgaaa
       61 gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcccagatct gggtacccaa
      121 gctaaacctc aaattccgaa agataaatcg aaagtggcag gctatattga aattccagat
      181 gctgatatta aagaaccagt atatccagga ccagcaacac gggaacaatt aaatagaggt
      241 gtaagctttg cagaagaaaa cgaatcacta gatgatcaaa atatttcaat tgcaggacac
      301 actttcattg accgtccgaa ctatcaattt acaaatctta aagcagccaa aaaaggtagt
      361 atggtgtact ttaaagttgg taatgaaaca cgtaagtata aaatgacaag tataagaaac
      421 gttaagccta cagccgtaga agttctagat gaacaaaaag gtaaagataa acaattaaca
      481 ttaattactt gtgatgatta caatgaagag acaggcgttt gggaaacccg taaaatcttt
      541 gtagctacag aagtcaaa
//
