LOCUS       sequence-109961-         504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA2woH
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..48
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt
       61 acccaagcta aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt
      121 ccagatgctg atattaaaga accagtatat ccaggaccag caacacctga acaattaaat
      181 agaggtgtaa gctttgcaaa ggaaaatgcc tcactagatg atcaaaatat ttcaattgca
      241 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      301 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      361 agagatgtta agcctacaga tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      421 ttaacattaa ttacttgtga tgattacaat gaaaagacag gcgtttggga aaaacgtaaa
      481 atctttgtag ctacagaagt caaa
//
