LOCUS       sequence-109965-         504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of 
            Staphylococcus aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA7woH
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..48
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt
       61 acccaagcta aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt
      121 ccagatgctg atattaaaga accagtatat ccaggaccag caacacggga acaattaaat
      181 agaggtgtaa gctttgcaaa ggaaaatgcc tcactagatg atcaaaatat ttcaattgca
      241 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      301 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      361 agaaacgtta agcctacagc cgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      421 ttaacattaa ttacttgtga tgattacaat gaagagacag gcgtttggga aacccgtaaa
      481 atctttgtag ctacagaagt caaa
//
