LOCUS       sequence-110002-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    471..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtacctt aatcactccg ggaagcaaga atttgcttca gcagccgatg gaaatactgc
      121 tgctgggagt tgaggtagcg cttgtactgt gacttgaagc ataactgccg gtacttgacc
      181 acctcggggt taaagtgtcc gtctcggaaa agcttctggg caatgtgcag agggtttcca
      241 aagcggaagt tctccccact gaacaaggct aagatgagtt cattctgctg ctcagcactg
      301 tcttcaggaa actggggcag aaactgctgg aggtgttcac gttgagaatc acttaacact
      361 tcctgccatg ttgagaggct gacaacatca aagaagatct caggatcctc cagaaggtcc
      421 tcgggcatat gtatatctcc ttcttatact taactaatat actaagatgg ggaattgtta
      481 tccgctcaca attcccctat agtgagtcgt attaatttcg attatgcggc cgtgtacaat
      541 acgattactt tctgttcgac ttaagcatta tgggccgcca ctgtgctgga tatctgcaga
      601 attccaccac actggactag tggatccgag ctcggtacct catttggttt cctgagcttt
      661 ctttgcgttc tgtttttctt ttgccttttc tactagtggt attaactgct ggtgttctgc
      721 taaagtcttt aacaattcca taatgaaagg cagataatta tgcttccttc tgatattctc
      781 aatcttgtat cttt
//
