LOCUS       sequence-110022-       14052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 14052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCaSpeR-act5cB-GAL4QF
FEATURES             Location/Qualifiers
     source          1..14052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     gene            complement(597..4725)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4698..4716
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     promoter        4846..7303
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
     CDS             7316..7756
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_feature    join(11060..11261,11262..11292)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(11829..11848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11948..11970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12008..12026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12094..12198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12199..12267,12268..13059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12417..12436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13230..13818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13719..13738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtaca tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg ttttttttat
     4681 ctactttccg caaaaatggg ttttattaac ttacatacat actagaattc tatattctaa
     4741 aaacacaaat gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt
     4801 tgcagatgca cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg
     4861 ttatgtggcc cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca
     4921 aacaccaaat acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct
     4981 tcgggcggag taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac
     5041 aatccaatgc caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg
     5101 cctggcgatc gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa
     5161 agactcgtcg gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg
     5221 ctgaggaatt tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta
     5281 tacgaaaatc agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg
     5341 caaaatatgt tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc
     5401 ttatgtattt ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaca
     5461 ggctgatttc tgcgtctttt gccgaacgca gtgcatgtgc ggttgttgtt ttttggaata
     5521 gtttcaattt tcggactgtc cgctttgatt tcagtttctt ggcttattca aaaagcaaag
     5581 taaagccaaa aaagcgagat ggcaatacca aatgcggcaa aacggtagtg gaaggaaagg
     5641 ggtgcggggc agcggaagga agggtggggc ggggcgtggc ggggtctgtg gctgggcgcg
     5701 acgtcaccga cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat tattcgtgtc
     5761 tcgccactcg ccggttgttt ttttcttttt atctcgctct ctctagcgcc atctcgtacg
     5821 catgctcaac gcaccgcatg ttgccgtgtc ctttatgcgt cattttggct cgaaataggc
     5881 aattatttaa acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt
     5941 tacttattgc catgtgtgtg cagccaacga tagcaacaaa agcaacaaca cagtggcttt
     6001 ccctctttca ctttttgttt gcaagcgcgt gcgagcaaga cggcacgacc ggcaaacgca
     6061 attacgctga caaagagcag acgaagtttt ggccgaaaaa catcaaggcg cctgatacga
     6121 atgcatttgc aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa
     6181 acacacaaaa aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacagctta
     6241 tcgttacggg ctaacagcac accgagacga aatagcttac ctgacgtcac agcctctgga
     6301 agaactgccg ccaagcagac gatgcagagg acgacacata gagtagcgga gtaggccagc
     6361 gtagtacgca tgtgcttgtg tgtgaggcgt ctctctcttc gtctcctgtt tgcgcaaacg
     6421 catagactgc actgagaaaa tcgattacct attttttatg aatgaatatt tgcactatta
     6481 ctattcaaaa ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc
     6541 gatgcaacga aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata
     6601 gaaatgcttc ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa
     6661 aaccggctta agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg
     6721 ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta
     6781 atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa taaacccact
     6841 ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt
     6901 aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac
     6961 ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg
     7021 atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa
     7081 aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga
     7141 gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc
     7201 gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga
     7261 cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat ccaacatgaa
     7321 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
     7381 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
     7441 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
     7501 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
     7561 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
     7621 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
     7681 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
     7741 acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa ttccgttgga
     7801 ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg acatgtcgga
     7861 tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg acggttctct
     7921 cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact ctaacgttaa
     7981 caggctcccg accatgatta cggatagata cacgttggct tctagatcca caacatcccg
     8041 tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc actcaccgac
     8101 gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat ggcaaatcct
     8161 ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta ctgatataga
     8221 tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg agtcaggttc
     8281 cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga ggcagaaaac
     8341 aaatactagc tataattttc acagcttttc cataagaatg gccatatcat tgggcttgaa
     8401 tagggacctc ccctcgtcct tcagtgatag cagcattctg gaacaaagac gccgaatttg
     8461 gtggtctgtc tactcttggg agatccaatt gtccctgctt tatggtcgat ccatccagct
     8521 ttctcagaat acaatctcct tcccttcttc tgtcgacgat gtgcagcgta ccacaacagg
     8581 tcccaccata tatcatggca tcattgaaac agcaaggctc ttacaagttt tcacaaaaat
     8641 ctatgaacta gacaaaacag taactgcaga aaaaagtcct atatgtgcaa aaaaatgctt
     8701 gatgatttgt aatgagattg aggaggtttc gagacaggca ccaaagtttt tacaaatgga
     8761 tatttccacc accgctctaa ccaatttgtt gaaggaacac ccttggctat cctttacaag
     8821 attcgaactg aagtggaaac agttgtctct tatcatttat gtattaagag attttttcac
     8881 taattttacc cagaaaaagt cacaactaga acaggatcaa aatgatcatc aaagttatga
     8941 agttaaacga tgctccatca tgttaagcga tgcagcacaa agaactgtta tgtctgtaag
     9001 tagctatatg gacaatcata atgtcacccc atattttgcc tggaattgtt cttattactt
     9061 gttcaatgca gtcctagtac ccataaagac tctactctca aactcaaaat cgaatgctga
     9121 gaataacgag accgcacaat tattacaaca aattaacact gttctgatgc tattaaaaaa
     9181 actggccact tttaaaatcc agacttgtga aaaatacatt caagtactgg aagaggtatg
     9241 tgcgccgttt ctgttatcac agtgtgcaat cccattaccg catatcagtt ataacaatag
     9301 taatggtagc gccattaaaa atattgtcgg ttctgcaact atcgcccaat accctactct
     9361 tccggaggaa aatgtcaaca atatcagtgt taaatatgtt tctcctggct cagtagggcc
     9421 ttcacctgtg ccattgaaat caggagcaag tttcagtgat ctagtcaagc tgttatctaa
     9481 ccgtccaccc tctcgtaact ctccagtgac aataccaaga agcacacctt cgcatcgctc
     9541 agtcacgcct tttctagggc aacagcaaca gctgcaatca ttagtgcgtc agttggagct
     9601 ccctcctacc gccacggcta cggcctcgat aatgccgcac gtgatggagc agcctctcag
     9661 taccagcatt aaccccgtca acgaccgctt caacggtatt cccaacccca ctccgtataa
     9721 ctccgatgca gctctcgatg ctatcactca gaccaacgat tatggaagcg taaatacaca
     9781 tggtatcctc tctacttacc cgccaccggc tacgcacctt aatgaagctt ccgtcgctct
     9841 cgctcccggt ggcgcccccc cccgaccgcc tcctccgtat gttgacagca cgaccaatca
     9901 cccgccgtac cactcgaatc tggttccaat ggcgaacttt ggttactcga ccgttgatta
     9961 cgatgccatg gttgacgatt tggctagcat tgaatacacg gacgctgtgg atgtcgaccc
    10021 acagtttatg accaatctgg gattcgttcc tggatgtaac ttctccgaca ttaatacata
    10081 caaaaagaag aaatgagcgg ccgcgatccg tcgaccatga agatcaagat cattgccccg
    10141 ccagagcgca agtactctgt ctggatcggt ggctccatcc tggcttcgct gtccaccttc
    10201 cagcagatgt ggatctccaa gcaggagtac gacgagtccg gcccctccat tgtgcaccgc
    10261 aagtgcttct aagaaggatc gcttgtctgg gcaagaggat caggatcggg atggtcttga
    10321 ttctgctgga ggaggaggag gagaagtcga ggaagcagca gcgaaagtgc aagtgcgagt
    10381 ggtggaagtt tggagtgcag cacaacaaaa tcaacaacaa caccaactac aagatgaaaa
    10441 gagcggaacc acctgcacac catcatcact atcatcatcg ttttgggcgc atgttgtgtg
    10501 gttccagcgt attaatataa ttaatttatt ccacatgaga tatgatatga tatactatgt
    10561 attttttgtt ttttttttat ttgtaaacct ttaatataac aagaactaca aaaaatgaaa
    10621 atgagcgaaa atgcatattc tgccattcca cacacacacc aacaacaccc aacacacgca
    10681 cacccacaag cttacacaca cacattcgcg gcatgacaag gacatcaaga taaagaagaa
    10741 cttaaagaag atatttccca aagcgcaaaa agaacacaca cacattgcaa aacacaaaca
    10801 acacactagc gttttgtaca attcgtcagc aaccttatgt attatttttt aattatgatg
    10861 taattataaa caaagtgaaa caaaaatatg aaaacaaaaa ggaaaatcaa atctgtcttc
    10921 tctttctccc gctctcctcg ctctctgctg ctaacctcgc cctctcctct ctcatctttt
    10981 tgtctgtctc tcttccacat ttttgccggc cggcaaaata ataacccaca cacactcaca
    11041 cttggctgca gccaagcttt gcgtactcgc aaattattaa aaataaaact ttaaaaataa
    11101 tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa gggttaatca
    11161 acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta ttcctttcac
    11221 tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac caccttatgt
    11281 tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
    11341 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
    11401 tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta
    11461 attatatttg tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg
    11521 aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat
    11581 accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac
    11641 atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt
    11701 tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct
    11761 tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg catatggtgc
    11821 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
    11881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    11941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
    12001 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
    12061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    12121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    12181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    12241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    12301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    12361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    12421 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
    12481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    12541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    12601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    12661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    12721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    12781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    12841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    12901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    12961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    13021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    13081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    13141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    13201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    13261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    13321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
    13381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    13441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    13501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    13561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    13621 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    13681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    13741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    13801 gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc agtatacctc
    13861 aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa
    13921 tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt
    13981 tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac
    14041 gtgggtctgg cc
//
