LOCUS       sequence-110071-        1661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian retroviral expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Kharas MG, Okabe R, Ganis JJ, Gozo M, Khandan T, Paktinat M, 
            Gilliland DG, Gritsman K
  TITLE     Constitutively active AKT depletes hematopoietic stem cells and 
            induces leukemia in mice.
  JOURNAL   Blood. 2010 Feb 18;115(7):1406-15. doi: 
            10.1182/blood-2009-06-229443. Epub 2009 Dec 14.
  PUBMED    20008787
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2009-06-229443"; journalName: "Blood"; date: 
            "2010-02-18- 18"; volume: "115"; issue: "7"; pages: "1406-15"
COMMENT     Sequence Label: pMSCV-flag-myr-Akt1-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..96
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             151..1587
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="SDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVD
                     QREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQT
                     VADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKV
                     ILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRL
                     CFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLML
                     DKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYE
                     MMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAK
                     EIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSME
                     CVDSERRPHFPQFSYSASSTA"
ORIGIN
        1 tttatcagcc ctcactcctt ctctaggcgc cggccggatc cagaattcgc caccatgggg
       61 tcttcaaaat ctaaaccaaa ggaccccagc cagcgccggc gcaggatccg aggttacctt
      121 gactacaaag acgatgacga caagcaattg agcgacgtgg ctattgtgaa ggagggttgg
      181 ctgcacaaac gaggggagta catcaagacc tggcggccac gctacttcct cctcaagaat
      241 gatggcacct tcattggcta caaggagcgg ccgcaggatg tggaccaacg tgaggctccc
      301 ctcaacaact tctctgtggc gcagtgccag ctgatgaaga cggagcggcc ccggcccaac
      361 accttcatca tccgctgcct gcagtggacc actgtcatcg aacgcacctt ccatgtggag
      421 actcctgagg agcgggagga gtggacaacc gccatccaga ctgtggctga cggcctcaag
      481 aagcaggagg aggaggagat ggacttccgg tcgggctcac ccagtgacaa ctcaggggct
      541 gaagagatgg aggtgtccct ggccaagccc aagcaccgcg tgaccatgaa cgagtttgag
      601 tacctgaagc tgctgggcaa gggcactttc ggcaaggtga tcctggtgaa ggagaaggcc
      661 acaggccgct actacgccat gaagatcctc aagaaggaag tcatcgtggc caaggacgag
      721 gtggcccaca cactcaccga gaaccgcgtc ctgcagaact ccaggcaccc cttcctcaca
      781 gccctgaagt actctttcca gacccacgac cgcctctgct ttgtcatgga gtacgccaac
      841 gggggcgagc tgttcttcca cctgtcccgg gaacgtgtgt tctccgagga ccgggcccgc
      901 ttctatggcg ctgagattgt gtcagccctg gactacctgc actcggagaa gaacgtggtg
      961 taccgggacc tcaagctgga gaacctcatg ctggacaagg acgggcacat taagatcaca
     1021 gacttcgggc tgtgcaagga ggggatcaag gacggtgcca ccatgaagac cttttgcggc
     1081 acacctgagt acctggcccc cgaggtgctg gaggacaatg actacggccg tgcagtggac
     1141 tggtgggggc tgggcgtggt catgtacgag atgatgtgcg gtcgcctgcc cttctacaac
     1201 caggaccatg agaagctttt tgagctcatc ctcatggagg agatccgctt cccgcgcacg
     1261 cttggtcccg aggccaagtc cttgctttca gggctgctca agaaggaccc caagcagagg
     1321 cttggcgggg gctccgagga cgccaaggag atcatgcagc atcgcttctt tgccggtatc
     1381 gtgtggcagc acgtgtacga gaagaagctc agcccaccct tcaagcccca ggtcacgtcg
     1441 gagactgaca ccaggtattt tgatgaggag ttcacggccc agatgatcac catcacacca
     1501 cctgaccaag atgacagcat ggagtgtgtg gacagcgagc gcaggcccca cttcccccag
     1561 ttctcctact cggccagcag cacggcctga ggcggcggtg gactgcggaa ttcactcgag
     1621 atacgtaaaa caattgaaga tctagtcgac caattccggt a
//
