LOCUS       sequence-110112-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human AID tagged with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Lambert LJ, Walker S, Feltham J, Lee HJ, Reik W, Houseley J
  TITLE     Etoposide induces nuclear re-localisation of AID.
  JOURNAL   PLoS One. 2013 Dec 4;8(12):e82110. doi: 
            10.1371/journal.pone.0082110. eCollection 2013.
  PUBMED    24324754
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-4"; volume: "8(12):e82110. doi"; pages: " 
            10.1371/journal.pone.0082110. eCollection 2013"
COMMENT     Sequence Label: pEGFP-AID
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..732)
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     primer_bind     complement(796..817)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcctatcaaa gtcccaaagt acgaaatgcg tctcgtaagt catcaacctc
      181 atacaggggc aaaaggatgc gccgaagctg tctggagaga cgaactgaat tttcatgcag
      241 cccttcccag gctttgaaag ttctttcatg gttttctaca aaagtattcc agcagtaaaa
      301 ataatctttg aaggtcatga tggctatttg caccccggcg cggtgcagcc gccgcagccc
      361 ctcgggctca gccttgcggt cctcacagaa gtagaggcgc gcggtgaaga tcctcagact
      421 gaggttgggg ttccctcgca gaaagtcggc cacatgtcgg gcacagtcgt agcaggggct
      481 ccaggaggtg aaccaggtga cgcggtagca gcggccaggg tctaggtccc agtccgagat
      541 gtagcggagg aagagcaatt ccacgtggca gccgttctta ttgcgaagat aaccaaagtc
      601 cagtgaaaag gatgtagcac tgtcacgcct cttcactacg tagcacaggt aggtctcacg
      661 ccgaccctta gcccagcgga catttttgaa ttggtaaaga aacttcctcc ggttcatcaa
      721 gaggctgtcc atagctcgag atctgagtcc ggacttgtac agctcgtcca tgccgagagt
      781 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      841 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      901 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      961 gtggcggatc t
//
