LOCUS       sequence-110116-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Lin TY, Voronovsky A, Raabe M, Urlaub H, Sander B, Golas MM
  TITLE     Dual tagging as an approach to isolate endogenous chromatin 
            remodeling complexes from Saccharomyces cerevisiae.
  JOURNAL   Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 
            10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5.
  PUBMED    25486077
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbapap.2014.11.009"; journalName: "Biochim Biophys Acta";
            date: "2015-03"; volume: "1854"; issue: "3"; pages: "198-208"
COMMENT     Sequence Label: pGS1044
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             240..305
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      331..518
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(386..410)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        593..936
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gctttctgct tctggttctg
      241 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
      301 acaagtaagt cgaccgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
      361 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      421 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      481 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
      541 gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      601 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      661 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      721 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
      781 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      841 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      901 atacagttct cacatcacat ccgaacataa acaaccatgt ctaagaatat cgttgtccta
      961 ccgggtgatc
//
