LOCUS       sequence-110120-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Strep tag II followed by TRP1 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1011
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..40)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     140..159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     353..375
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     367..384
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     368..384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             412..435
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(470..486)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..486)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
       61 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      121 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
      181 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
      241 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      301 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      361 cgacgttgta aaacgacggc cagtgaattc gagctcggta ccggtagtgc ttggagtcat
      421 ccacaattcg aaaaataaaa gcttgatatc gaattcctgc agcccggggg atccactagt
      481 tctagagcgg ccgcggaacg atcattcact atatatatat caatttatat atacgtatgt
      541 gtaattgaag aaagatacgt tttttcctct attgagaggc ctgctggatg aatagcttta
      601 ccttttctaa atccttgata ccatcagtct ctactcctcc acttacatcg acaccgatcg
      661 catttggtaa catattaatg gcaacagaaa cgttatcagg attcaatcca ccagcgataa
      721 tgaattttat ctcgggatga cttgcagacc aactggaaat tgcactccaa ttcaatttct
      781 caccagtgcc accttcacca gaatcgaaca acgtcagcac attgtctacg tgttcataca
      841 ggtccagtag taattcacaa tcctgtggga actggaacct cttaa
//
