LOCUS       sequence-110129-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by 
            HIS5 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1623
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(88..285)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(291..944)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
ORIGIN
        1 ggccgcatag gccactagtg gatctgatat catcgatgaa ttcgagctcg tttaaactgg
       61 atggcggcgt tagtatcgaa tcgacagcag tatagcgacc agcattcaca tacgattgac
      121 gcatgatatt actttctgcg cacttaactt cgcatctggg cagatgatgt cgaggcgaaa
      181 aaaaatataa atcacgctaa catttgatta aaatagaaca actacaatat aaaaaaacta
      241 tacaaatgac aagttcttga aaacaagaat ctttttattg tcagtactct ttacaacact
      301 cccttcgtgc ttgggacttc agaacttcca gtaagactag tagccgcgcg catggcaaca
      361 gccagagatt taaaagcgct ttcagcacga tgatggtcat tactaccata taagcaggta
      421 acatgcaaag taattccagc tgctaccgaa aaggaatata gtaagtgagg gatcatttca
      481 caggacaatt ccccaacctt ttcacgcttt aatcccaaat cgataacagc atagggccgt
      541 cccgacaagt caactacgct tctagaaaga gcttcgtcaa gtggacaata agcatgtcca
      601 aatcttttaa cgccggcaaa gttactcata gcctgcttga atgcaatacc aagtgcaata
      661 gcagtatctt ctgcagtgtg atgatcatcg atgattaaat cacctcttga gtaaagtcgt
      721 aagctccagc ctgcatgttt agccagtgca tgatacatgt gatccaagaa tccaattccc
      781 gtgtctactt ggattacttg ttctcccttt tggtttgtat gcttggaagt tataagttca
      841 tcaataaaat tcgactcttc aggtaaggga gctttgtcca aagcgatggc aacgctgatt
      901 ttcgtttcgt tcgtatttct ttctacaaaa gccctcctac ccatggttgt ttatgttcgg
      961 atgtgatgtg agaactgtat cctagcaaga ttttaaaagg aagtatatga aagaagaacc
//
