LOCUS       sequence-110130-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by 
            HIS5 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1623
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..727
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(183..201)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(233..253)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     451..470
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     645..668
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca tggtggcctc
       61 ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      181 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      241 ccagttccag tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgactacaa
      301 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
      421 gttccgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
      481 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
      541 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
      601 caagaagccc gtgcagctgc ccggctacta ctacgtggac accaagctgg acatcacctc
      661 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
      721 gttcctggg
//
