LOCUS       sequence-110132-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by 
            HIS5 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1623
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(72..95,96..131,132..155,156..161))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             complement(165..836)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(224..247)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(422..441)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     639..659
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     691..709
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 gcggtacctt acttctcaaa ttgtggatga gaccaggcag aacctcccga tccacctccg
      121 gaacctccac ctttttcgaa ttgtggatga ctccaagcac tacccaggaa caggtggtgg
      181 cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      241 tccagcttgg tgtccacgta gtagtagccg ggcagctgca cgggcttctt ggccatgtag
      301 atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc ctggtggatc
      361 tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccatg
      421 gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcggaa cttcaccttg
      481 tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
      541 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt cttgtagtcg
      601 gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt actggaactg gggggacagg
      661 atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg
      721 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
      781 ccctccatgc gcaccttgaa gcgcatgaac tctttgatga cgtcctcgga ggaggcggtg
      841 ccggagctgc cgctgccggt gctgccggtg ccatgcccca ggaacaggtg gtggcggccc
      901 tcggagcgct cgtactgttc cacga
//
