LOCUS       sequence-110135-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1222
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(94..291)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgcggccgca taggccacta gtggatctga tatcatcgat gaattcgagc tcgttttcga
       61 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      121 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      181 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      241 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tacagattat
      301 aattggccag tctttttcaa ataagcattc caaccagcat ctctatacct tttaccttca
      361 atatctggat ctcttccttt accaaacaat cctctaccaa caatgataat atcagttcca
      421 gtgctaacaa cttcatcaac agttctatat tgttgtccta atccatcacc tttatcatct
      481 aatccaactc caggtgtcat aataagccaa tcaaatcctt cttcttgtcc acccatatca
      541 cgttgggcaa taaatccaat aacaaattcc ttatcggatt tagcaatttc aacagttttt
      601 tgagaatatt ctccatatgc taatgatccc actgatgata attcagctaa catcaataac
      661 cctcttggct cttggttggt ggtggtttct ttagctccct gttttaatcc ttcaactact
      721 ccattcccag tgacaccatg agcattagta atatctgccc aactactaat tttataaact
      781 ccaccaatat attgtttctt cacggtatta ccaatatcag caaattttct atcttcaaaa
      841 atcataaatt gatgtttacg tgaaagttct aataatggtt caatagtgga ttcataggaa
      901 aaatcattga ttatatcaat atgagtcttg attaagcata c
//
