LOCUS       sequence-110136-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1222
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..769
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             56..769
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(773..778,779..802,803..838,839..862)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 cgaagcttcg tacgctgcag gtcgacggat cggtgacggt gctggtttaa ttaacatgtc
       61 taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt
      121 taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt
      181 gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac
      241 tactttaact tatggtgttc aatgtttttc tagataccca gatcatatga aacaacatga
      301 ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga
      361 tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag
      421 aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga
      481 atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa
      541 agttaacttc aaaattagac acaacattga agatggttct gttcaattag ctgaccatta
      601 tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc
      661 cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga
      721 atttgttact gctgctggta ttacccatgg tatggatgaa ttgtacaaag gtagtgcttg
      781 gagtcatcca caattcgaaa aaggtggagg ttccggaggt ggatcgggag gttctgcctg
      841 gtctcatcca caatttgaga agtaaggtac cgcgcgcgcc acttctaaat aagcgaattt
      901 cttatgattt atgattttta ttattaaata agttataaaa a
//
