LOCUS       sequence-110140-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1212
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..763
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             50..763
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             773..796
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca tgtctaaagg
       61 tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg
      121 tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt
      181 aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt
      241 aacttatggt gttcaatgtt tttctagata cccagatcat atgaaacaac atgacttttt
      301 caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg
      361 taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga
      421 attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa
      481 ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa
      541 cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca
      601 aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatccactca
      661 atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt tagaatttgt
      721 tactgctgct ggtattaccc atggtatgga tgaattgtac aaaggtagtg cttggagtca
      781 tccacaattc gaaaaataag gtaccgcgcg cgccacttct aaataagcga atttcttatg
      841 atttatgatt tttattatta aataagttat aaaaaaa
//
