LOCUS       sequence-110151-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1613
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(294..947)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
ORIGIN
        1 cgcggccgca taggccacta gtggatctga tatcatcgat gaattcgagc tcgtttaaac
       61 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tctttacaac
      301 actcccttcg tgcttgggac ttcagaactt ccagtaagac tagtagccgc gcgcatggca
      361 acagccagag atttaaaagc gctttcagca cgatgatggt cattactacc atataagcag
      421 gtaacatgca aagtaattcc agctgctacc gaaaaggaat atagtaagtg agggatcatt
      481 tcacaggaca attccccaac cttttcacgc tttaatccca aatcgataac agcatagggc
      541 cgtcccgaca agtcaactac gcttctagaa agagcttcgt caagtggaca ataagcatgt
      601 ccaaatcttt taacgccggc aaagttactc atagcctgct tgaatgcaat accaagtgca
      661 atagcagtat cttctgcagt gtgatgatca tcgatgatta aatcacctct tgagtaaagt
      721 cgtaagctcc agcctgcatg tttagccagt gcatgataca tgtgatccaa gaatccaatt
      781 cccgtgtcta cttggattac ttgttctccc ttttggtttg tatgcttgga agttataagt
      841 tcatcaataa aattcgactc ttcaggtaag ggagctttgt ccaaagcgat ggcaacgctg
      901 attttcgttt cgttcgtatt tctttctaca aaagccctcc tacccatggt tgtttatgtt
      961 cggatgtgat gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga
     1021 acctc
//
