LOCUS       sequence-110155-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for solubility screening of proteins or protein 
            fragments using a split-GFP assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Bulloch EM, Kingston RL
  TITLE     Identifying protein domains by global analysis of soluble fragment 
            data.
  JOURNAL   Anal Biochem. 2014 Jul 10. pii: S0003-2697(14)00280-2. doi: 
            10.1016/j.ab.2014.06.021.
  PUBMED    25016192
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Jul 10. pii: S0003-2697(14)00280-2. doi: 
            10.1016/j.ab.2014.06.021."
COMMENT     Sequence Label: pBAD_GFP11_T7LysH17A
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..23
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(83..130)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(188..205)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(251..535)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(309..328)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagccaagct
       61 cacgagtgcg gccgcaagct tatgtgatgc cagcagcatt tacgtactca tgaagaacca
      121 tatggtcacg acttgttgaa ccagaaccag agcctccatc ctcgagcagc tgactagtgc
      181 taccaccatg atgatgatga tgatgagaac cgcccatggt taattcctcc tgttagccca
      241 aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct
      301 aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg
      361 tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc
      421 ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc
      481 cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg
      541 aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac
      601 tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat
      661 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt
      721 cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg
      781 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac
      841 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata
      901 tttgccaata cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg
      961 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata
     1021 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca
     1081 ccgatggata atcgggtac
//
