LOCUS       sequence-110169-        2351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK009
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..693
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     563..586
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      complement(716..750)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(821..1480)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1395..1414
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1662..2250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2151..2170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta
       61 aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc
      121 tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta
      181 ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta
      241 agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca
      301 tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa
      361 aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc
      421 aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct
      481 ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac
      541 ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt
      601 aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac
      661 ttagtttcga ataaacacac ataaacaaac aaaagatcta tgtgagacca gaccaataaa
      721 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      781 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      841 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      901 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      961 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1021 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     1081 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1141 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1201 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1261 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1321 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1381 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1441 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1501 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1561 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     1621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1801 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2281 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2341 gataaccgta g
//
