LOCUS       sequence-110172-        2347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pHHF2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK012
FEATURES             Location/Qualifiers
     source          1..2347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(712..746)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(817..1476)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1391..1410
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1658..2246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2147..2166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtgtggag tgtttgcttg gattctttag taaaagggga agaacagttg
       61 gaagggccaa agtggaagtc acaaaacagt ggtcctatat aaaagaacaa gaaaaagatt
      121 atttatatac aactgcggtc acaagaagca acgcgagaga gcacaacacg ctgttatcac
      181 gcaaactatg ttttgacacc gagccatagc cgtgattgtg cgtcacattg ggcgataatg
      241 aacgctaaat gaccaactcc catccgtagg agccccttag ggcgtgccaa tagtttcacg
      301 cgcttaatgc gaagtgctcg gaacggacaa ctgtggtcgt ttggcaccgg gaaagtggta
      361 ctagaccgag agtttcgcat ttgtatggca ggacgttctg ggagcttcgc gtctaaagct
      421 ttttcgggcg cgaaatgcag accagaccag aacaaaacaa ctgacaagaa ggcgtttaat
      481 ttaatatgtt gttcactcgc gcctgggctg ttgttattcg gctagataca tacgtgtttg
      541 tgcgtatgta gttatatcat atataagtat attaggatga ggcggtgaaa gagatttttt
      601 ttttttcgct taatttattc ttttctctat cttttttcct acatcttgtt caaaagagta
      661 gcaaaaacaa caatcaatac aataaaataa gatctatgtg agaccagacc aataaaaaac
      721 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      781 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      841 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      901 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      961 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1021 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1081 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1141 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1201 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1261 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1321 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1381 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1441 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     1501 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1561 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     1621 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1681 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1741 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2341 accgtag
//
