LOCUS       sequence-110176-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pHTB2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK016
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(735..769)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(840..1499)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1414..1433
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1681..2269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2170..2189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtatatat taaatttgct cttgttctgt actttcctaa ttcttatgta
       61 aaaagacaag aatttatgat actatttaat aacaaaaaac tacctaagaa aagcatcatg
      121 cagtcgaaat tgaaatcgaa aagtaaaact ttaacggaac atgtttgaaa ttctaagaaa
      181 gcatacatct tcatccctta tatatagagt tatgtttgat attagtagtc atgttgtaat
      241 ctctggccta agtatacgta acgaaaatgg tagcacgtcg cgtttatggc ccccaggtta
      301 atgtgttctc tgaaattcgc atcactttga gaaataatgg gaacacctta cgcgtgagct
      361 gtgcccaccg cttcgcctaa taaagcggtg ttctcaaaat ttctccccgt tttcaggatc
      421 acgagcgcca tctagttctg gtaaaatcgc gcttacaaga acaaagaaaa gaaacatcgc
      481 gtaatgcaac agtgagacac ttgccgtcat atataaggtt ttggatcagt aaccgttatt
      541 tgagcataac acaggttttt aaatatatta ttatatatca tggtatatgt gtaaaatttt
      601 tttgctgact ggttttgttt atttatttag ctttttaaaa attttacttt cttcttgtta
      661 attttttctg attgctctat actcaaacca acaacaactt actctacaac taagatctat
      721 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      781 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      841 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      901 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      961 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1021 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     1081 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1141 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1201 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1261 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1321 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1381 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1441 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1501 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1561 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2341 gttatcccct gattctgtgg ataaccgtag
//
