LOCUS       sequence-110177-        2371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pRPL18B as a Type 2 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK017
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(736..770)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(841..1500)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1415..1434
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1682..2270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2171..2190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgaagagga tgtccaatat tttttttaag gaataaggat acttcaagac
       61 tagattcccc cctgcattcc catcagaacc gtaaaccttg gcgctttcct tgggaagtat
      121 tcaagaagtg ccttgtccgg tttctgtggc tcacaaacca gcgcgcccga tatggctttc
      181 ttttcactta tgaatgtacc agtacgggac aattagaacg ctcctgtaac aatctctttg
      241 caaatgtggg gttacattct aaccatgtca cactgctgac gaaattcaaa gtaaaaaaaa
      301 atgggaccac gtcttgagaa cgatagattt tctttatttt acattgaaca gtcgttgtct
      361 cagcgcgctt tatgttttca ttcatacttc atattataaa ataacaaaag aagaatttca
      421 tattcacgcc caagaaatca ggctgctttc caaatgcaat tgacacttca ttagccatca
      481 cacaaaactc tttcttgctg gagcttcttt taaaaaagac ctcagtacac caaacacgtt
      541 acccgacctc gttattttac gacaactatg ataaaattct gaagaaaaaa taaaaaaatt
      601 ttcatacttc ttgcttttat ttaaaccatt gaatgatttc ttttgaacaa aactacctgt
      661 ttcaccaaag gaaatagaaa gaaaaaatca attagaagaa aacaaaaaac aaaagatcta
      721 tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      781 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      841 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      901 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      961 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1021 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
     1081 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1141 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1201 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1261 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1321 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1381 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1441 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1501 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1561 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
     1621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2341 cgttatcccc tgattctgtg gataaccgta g
//
