LOCUS       sequence-110188-        2371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pMFA1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK028
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(736..770)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(841..1500)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1415..1434
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1682..2270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2171..2190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcgctaga aggaaattgg aagaactaaa tagagttctg aaaaacaatc
       61 catatttcaa ttcaaatgtg aacgtacaca attcgagact caaagatgct gtaccgttca
      121 cgccgtttaa cggtgataga gaagcacacc caaggtttac gttgaaaggt tcagtataca
      181 atgacccatt catcaaacat cttgagcaca ggaaagaatt tattgcgtct gggttcaaca
      241 ctaattatgc gtacgaaagg gtgttgacag aggcatttat gggcttagga tgtgttatat
      301 ccgaggagct ttaaaacatc aggatagtgt gcaacgtggc ataagctatg taatcaacta
      361 ctttttattt tctatgtacg catatacatg cattcacgat ctgtttcagt gttcagaaaa
      421 aaggcaccta ctgctacggt tggcccatac ctttattctt tgttcttgtt acaaacgagt
      481 gtgtaattac ccaaaaagga aatttacatg ttaaatgaaa cccagtaatc agaaaaaaca
      541 gttaagaaac ctaaaatggt agagataaag atacagattc agtggttgct gaaaatcaag
      601 taaaaaaatg aaatagagtc atcatatata aaccgccaga aatgaattaa tgagagggat
      661 ctgtaactgt ttctcggata aaaccaaaat aagtacaaag ccatcgaata gaaagatcta
      721 tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      781 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      841 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      901 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      961 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1021 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
     1081 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1141 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1201 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1261 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1321 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1381 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1441 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1501 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1561 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
     1621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2341 cgttatcccc tgattctgtg gataaccgta g
//
