LOCUS       sequence-110189-        2421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pMF_2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK029
FEATURES             Location/Qualifiers
     source          1..2421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(786..820)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(891..1550)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1465..1484
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1732..2320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2221..2240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgttgatct gccttttata gctaagaaaa aaaaagagaa aaaaataata
       61 agtgcgggta acctcatgca ttgtaaaacg gtagggctac aaggcactac ttactctacg
      121 ctatagaaga agcttcaaga catggtgttg ttcttatttg agcgaactca gctgtcaccg
      181 tttcgggaaa ctctatagtt ttctgcgttt cagtacgcag ttgggcgtgc taaagttgtt
      241 ttcctaattt gctagtgttc acttgctcat tgatgtccct aaaaggtaat gtcgcatagt
      301 aagatgtatg tcccatatca aacgcattcc tagaaaaaca tgcactgaat gaataagcag
      361 ttacttcgat tactaatctt cacagcataa tttttctctt atcttgtaaa cctctttcta
      421 tcttttcata aagtttgtca cttatatttc ttcattggta catcaatgcc agcaacgatg
      481 tgcgcatctg ggcgacgcct gtagtgattg ttttcaaggt atcgagccaa actattcatc
      541 gttactgttt caaatattca gttgtttcag tacagagtcg ccgtggacct agtgaaactt
      601 ggtgtcttta cagcgcagag atgagggctt atatgtataa aagctgtcct tgattctggt
      661 gtagtttgag gtgtccttcc tatatctgtt tttatattct atataatgga taattactac
      721 catcacctgc atcaaattcc agtaaattca catattggag aaaagatcta tgtgagacca
      781 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      841 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      901 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      961 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1021 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1081 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1141 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1201 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1261 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1321 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1381 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1441 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1501 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1561 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1621 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2401 tgattctgtg gataaccgta g
//
