LOCUS       sequence-110190-        2204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK030
FEATURES             Location/Qualifiers
     source          1..2204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        94..535
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    94..211
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     506..529
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      complement(569..603)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(674..1333)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1248..1267
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1515..2103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2004..2023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgccccatt atcttagcct aaaaaaacct tctctttgga actttcagta
       61 atacgcttaa ctgctcattg ctatattgaa gtacggatta gaagccgccg agcgggcgac
      121 agccctccga cggatgactc tcctccgtgc gtcctcgtca tcaccggtcg cgttcctgaa
      181 acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt
      241 tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc aaattaacga
      301 atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta
      361 attaatcagc gaagcgatga tttttgatct attaacagat atataaatgg aaaagctgca
      421 taaccacttt aactaatact ttcaacattt tcagtttgta ttacttctta ttcaaatgtc
      481 ataaaagtat caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa
      541 actataagat ctatgtgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
      601 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
      661 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      721 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      781 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      841 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
      901 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      961 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1021 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1081 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1141 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1201 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1261 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1321 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1381 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc
     1441 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1501 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1561 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1621 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1681 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1741 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1801 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1861 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1921 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1981 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2041 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2101 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2161 atgttctttc ctgcgttatc ccctgattct gtggataacc gtag
//
