LOCUS       sequence-110192-        2382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mTurquoise2 as a Type 3 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK032
FEATURES             Location/Qualifiers
     source          1..2382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(747..781)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(852..1511)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1426..1445
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1693..2281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2182..2201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggtttcta aaggtgaaga attattcact ggtgttgtcc caattttggt
       61 tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag gtgaaggtga
      121 tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat tgccagttcc
      181 atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa gatacccaga
      241 tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag
      301 aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca agtttgaagg
      361 tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag atggtaacat
      421 tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca ctgctgacaa
      481 acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag atggtggtgt
      541 tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag tcttgttacc
      601 agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg aaaagaggga
      661 ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta tggatgaatt
      721 gtacaaagga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
      781 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
      841 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
      901 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
      961 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1021 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     1081 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1141 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1201 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1261 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1321 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1381 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1441 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1501 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1561 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     1621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2341 gttctttcct gcgttatccc ctgattctgt ggataaccgt ag
//
