LOCUS       sequence-110203-        2013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Ubi-R as a Type 3a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK043
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             11..238
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      complement(378..412)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(483..1142)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1057..1076
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1324..1912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1813..1832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgcagattt tcgttaaaac tttaactggt aaaacaatta ctttagaagt
       61 tgagtcatct gatactatcg ataatgttaa atctaagatc caggataagg aaggtattcc
      121 tccagatcaa caaagattga ttttcgcagg taagcagttg gaggacggta ggactttgtc
      181 tgattataac atacagaaag aatctacttt gcacttagtc ttaagattaa ggggaggtag
      241 acatggttct ggtgcctggt tgttacctgt atcattggtt aaaagaaaga ccacattggc
      301 accaaatact caaactgcct ctccaagagc cttggcagac tctttaatgc agagatcagg
      361 ttcttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      421 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      481 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      541 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      601 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      661 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
      721 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      781 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      841 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      901 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      961 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1021 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1081 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1141 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1201 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1441 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1981 tgcgttatcc cctgattctg tggataaccg tag
//
