LOCUS       sequence-110209-        1684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes I-SceI recognition site as a Type 234 part to be used in the
            Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK049
FEATURES             Location/Qualifiers
     source          1..1684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(49..83)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(154..813)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     728..747
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      995..1583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1484..1503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtagggat aacagggtaa tgctgtgaga ccagaccaat aaaaaacgcc
       61 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      121 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      181 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      241 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      301 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      361 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      421 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      481 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      541 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      601 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      661 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
      721 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
      781 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
      841 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
      901 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1141 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1681 gtag
//
