LOCUS       sequence-110214-        1900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tPGK1 as a Type 4 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK054
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(265..299)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(370..1029)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     944..963
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1211..1799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1700..1719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcctaactcg agattgaatt gaattgaaat cgatagatca atttttttct
       61 tttctctttc cccatccttt acgctaaaat aatagtttat tttatttttt gaatattttt
      121 tatttatata cgtatatata gactattatt tatcttttaa tgattattaa gatttttatt
      181 aaaaaaaaat tcgctcctct tttaatgcct ttatgcagtt tttttttccc attcgatatt
      241 tctatgtgct gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      361 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      421 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      481 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      541 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
      601 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      661 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      721 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      781 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
      841 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      901 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      961 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1021 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1081 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     1141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1861 tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
