LOCUS       sequence-110217-        2389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mTurquoise2 as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK057
FEATURES             Location/Qualifiers
     source          1..2389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(754..788)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(859..1518)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1433..1452
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1700..2288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2189..2208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgtttcta aaggtgaaga attattcact ggtgttgtcc caattttggt
       61 tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag gtgaaggtga
      121 tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat tgccagttcc
      181 atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa gatacccaga
      241 tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag
      301 aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca agtttgaagg
      361 tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag atggtaacat
      421 tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca ctgctgacaa
      481 acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag atggtggtgt
      541 tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag tcttgttacc
      601 agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg aaaagaggga
      661 ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta tggatgaatt
      721 gtacaaataa ctcgagtggc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc
      781 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      841 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
      901 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      961 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     1021 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     1081 tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1141 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1201 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1261 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     1321 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1381 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1441 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1501 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1561 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     1621 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
