LOCUS       sequence-110218-        2386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Venus as a Type 4a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK058
FEATURES             Location/Qualifiers
     source          1..2386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..727
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(751..785)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(856..1515)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1430..1449
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1697..2285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2186..2205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcctctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
       61 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
      121 tacttacggt aaattgacct taaaattgat ttgtactact ggtaaattgc cagttccatg
      181 gccaacctta gtcactactt taggttatgg tttgcaatgt tttgctagat acccagatca
      241 tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
      301 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
      361 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaaggtg gtaacatttt
      421 aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcactg ctgacaaaca
      481 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
      541 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
      601 caaccattac ttatcctatc aatctgcctt atccaaagat ccaaacgaaa agagagatca
      661 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
      721 caaataactc gagtggctga gaccagacca ataaaaaacg cccggcggca accgagcgtt
      781 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      841 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      901 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      961 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1021 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1081 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1141 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1201 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1261 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1321 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1381 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1441 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1501 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1561 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     1621 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1681 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1741 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1801 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1861 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1921 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1981 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2041 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2101 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2161 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2221 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2281 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2341 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
